human prostate cancer complete growth medium Search Results


human prostate epithelial cells cloneticstm prostate epithelial cell growth medium  (Lonza)
90
Lonza human prostate epithelial cells cloneticstm prostate epithelial cell growth medium
Top functional annotations enriched among macroH2A1-regulated genes
Human Prostate Epithelial Cells Cloneticstm Prostate Epithelial Cell Growth Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human prostate epithelial cells cloneticstm prostate epithelial cell growth medium/product/Lonza
Average 90 stars, based on 1 article reviews
human prostate epithelial cells cloneticstm prostate epithelial cell growth medium - by Bioz Stars, 2026-03
90/100 stars
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complete medium human prostatic epithelial cells  (Procell Inc)
90
Procell Inc complete medium human prostatic epithelial cells
Top functional annotations enriched among macroH2A1-regulated genes
Complete Medium Human Prostatic Epithelial Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete medium human prostatic epithelial cells/product/Procell Inc
Average 90 stars, based on 1 article reviews
complete medium human prostatic epithelial cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Top functional annotations enriched among macroH2A1-regulated genes

Journal: Nature structural & molecular biology

Article Title: MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation

doi: 10.1038/nsmb.2903

Figure Lengend Snippet: Top functional annotations enriched among macroH2A1-regulated genes

Article Snippet: Human prostate epithelial cells (Lonza) were cultured in CloneticsTM Prostate Epithelial Cell Growth Medium.

Techniques: Functional Assay

MacroH2A1 directly regulates the acetylation of H2B at K12 and K120 in primary cell types but not in cancer cells. (a) Immunoblots for macroH2A1 (mH2A1), histone H3 (H3) and the indicated histone PTMs in IMR90 cells expressing an shRNA against either Luciferase (Luc) as a control or macroH2A1. (b) Immunoblots for macroH2A1, H3 and the indicated histone marks in primary lung and skin fibroblasts, primary hepatocytes, primary prostate and mammary epithelial cells, A549 lung cancer cells, HeLa cervical carcinoma cells, HEK293T transformed embryonic kidney cells, HepG2 hepatocellular carcinoma cells and MCF-7 breast cancer cells expressing an shRNA against either Luciferase (L, as a control) or macroH2A1 (M). (c) ChIP-qPCR for H2BK12ac, H2BK120ac, macroH2A1 and histone H3 from IMR90 cells expressing an shRNA directed against either Luciferase (Luc, as a control) or macroH2A1 at H2BK12ac and H2BK120ac positive regions who are either positive (left) or negative (right) for macroH2A1 chromatin incorporation. The horizontal dotted line indicates upper limit of the 95% confidence interval of the signal from no-antibody negative control ChIPs. Error bars, +/− s.e.m. (n = 3 independent cell passages). *p < 0.05 from two-tailed Student’s t-tests. (d) Immunoprecipitation with antibodies directed against the indicated PTMs or no antibody (NA) as a control of mononucleosomes from either IMR90 primary lung fibroblasts or A549 lung cancer cells. Immunoblots were performed for both macroH2A1 and histone H3.

Journal: Nature structural & molecular biology

Article Title: MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation

doi: 10.1038/nsmb.2903

Figure Lengend Snippet: MacroH2A1 directly regulates the acetylation of H2B at K12 and K120 in primary cell types but not in cancer cells. (a) Immunoblots for macroH2A1 (mH2A1), histone H3 (H3) and the indicated histone PTMs in IMR90 cells expressing an shRNA against either Luciferase (Luc) as a control or macroH2A1. (b) Immunoblots for macroH2A1, H3 and the indicated histone marks in primary lung and skin fibroblasts, primary hepatocytes, primary prostate and mammary epithelial cells, A549 lung cancer cells, HeLa cervical carcinoma cells, HEK293T transformed embryonic kidney cells, HepG2 hepatocellular carcinoma cells and MCF-7 breast cancer cells expressing an shRNA against either Luciferase (L, as a control) or macroH2A1 (M). (c) ChIP-qPCR for H2BK12ac, H2BK120ac, macroH2A1 and histone H3 from IMR90 cells expressing an shRNA directed against either Luciferase (Luc, as a control) or macroH2A1 at H2BK12ac and H2BK120ac positive regions who are either positive (left) or negative (right) for macroH2A1 chromatin incorporation. The horizontal dotted line indicates upper limit of the 95% confidence interval of the signal from no-antibody negative control ChIPs. Error bars, +/− s.e.m. (n = 3 independent cell passages). *p < 0.05 from two-tailed Student’s t-tests. (d) Immunoprecipitation with antibodies directed against the indicated PTMs or no antibody (NA) as a control of mononucleosomes from either IMR90 primary lung fibroblasts or A549 lung cancer cells. Immunoblots were performed for both macroH2A1 and histone H3.

Article Snippet: Human prostate epithelial cells (Lonza) were cultured in CloneticsTM Prostate Epithelial Cell Growth Medium.

Techniques: Western Blot, Expressing, shRNA, Luciferase, Control, Transformation Assay, ChIP-qPCR, Negative Control, Two Tailed Test, Immunoprecipitation